P 212 Fluorophore identification — New algorithms

&&&s: A new instnunent has bee” developed, in order to increase the information about the mechanisms involved, which performs spectral analysis of the fluorescence light. This instlwnentation is though to be capable of doiig in viva (lens and anterior chamber) and in vifro measurements. The experimental system consist basically of: a modified slit lamp, with four different excitation wavelengths (36.5,404,436 and 485 mn), a spectrograph and a light detector. The whole system is controlled by a computer that also acquires and represents the spectra. Care has been taken not to use a light intensity higher than the safety illumination maximum oftbe reti”a.